Human IL-4 protein | PRP100273

Human IL-4 protein | PRP100273 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from mature form of human IL-4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified, with an initial Met.

Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg.

protein Lenght: The recombinant human IL-4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.

Preparation: E. coli

Purity: > 92 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile 20mM MES, 200mM NaCl, pH 6.0

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 15 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL4 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-4 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.70% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Interleukin-4, also known as IL-4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL-4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL-4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL-4 receptor α, completely inhibites in a dose-dependent manner the IL-4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL-4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL-4-stimulated BA/F3 cells.

Alternative Names: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Interleukin-4; Interleukin-4; IL4

Search Names: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Interleukin-4; Interleukin-4; IL4

Tag: IL-4