Human IL18R1 (Interleukin 18 Receptor 1) ELISA Kit | ELK3472

Human IL18R1 (Interleukin 18 Receptor 1) ELISA Kit | ELK3472 | Gentaur UK, US & Europe Distribution

Alternative Names: IL18-R1;CDw218a; CD218a;IL-1Rrp; IL1RRP; CD218 antigen-like family member A; IL1 receptor-related protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Receptor 1 (IL18R1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 18 Receptor 1 (IL18R1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 Receptor 1 (IL18R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 Receptor 1 (IL18R1) in the samples is then determined by comparing the OD of the samples to the standard curve.