Human IL18RAP (Interleukin 18 Receptor Accessory Protein) ELISA Kit | ELK3473

Human IL18RAP (Interleukin 18 Receptor Accessory Protein) ELISA Kit | ELK3473 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-1R7;CDw218b; CD218-B;ACPL; Accessory protein-likeL; CD218 antigen-like family member B; IL-1R accessory protein-like; Interleukin-1 receptor 7; Interleukin-18 receptor beta

Species: Human

Assay Type: Sandwich

Sensitivity: 0.051 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Receptor Accessory Protein (IL18RAP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 18 Receptor Accessory Protein (IL18RAP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 Receptor Accessory Protein (IL18RAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 Receptor Accessory Protein (IL18RAP) in the samples is then determined by comparing the OD of the samples to the standard curve.