Human IL1h (Interleukin 1 Eta) ELISA Kit | ELK3913

Human IL1h (Interleukin 1 Eta) ELISA Kit | ELK3913 | Gentaur UK, US & Europe Distribution

Alternative Names: IL1F8; IL36b; FIL1; FIL1- (ETA) ; FIL1H; IL-1F8; IL-1H2; IL1-ETA; IL1H2; Interleukin 36, Beta; Interleukin-1 Family Member 8; Interleukin-1 homolog 2

Species: Human

Assay Type: Sandwich

Sensitivity: 2.7 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Eta (IL1h) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Eta (IL1h) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Eta (IL1h), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Eta (IL1h) in the samples is then determined by comparing the OD of the samples to the standard curve.