Human IL1RAPL2 (Interleukin 1 Receptor Accessory Protein Like Protein 2) ELISA Kit | ELK3221

Human IL1RAPL2 (Interleukin 1 Receptor Accessory Protein Like Protein 2) ELISA Kit | ELK3221 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-1R9; IL1R9; IL1RAPL-2; TIGIRR-1; IL1RAPL-2-related protein; Interleukin-1 receptor 9; Three immunoglobulin domain-containing IL-1 receptor-related 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) in the samples is then determined by comparing the OD of the samples to the standard curve.