Human IL1RL1 (Interleukin 1 Receptor Like Protein 1) ELISA Kit | ELK4866

Human IL1RL1 (Interleukin 1 Receptor Like Protein 1) ELISA Kit | ELK4866 | Gentaur UK, US & Europe Distribution

Alternative Names: T1; ST2; DER4; FIT-1; ST2L; ST2V; Homolog Of Mouse Growth Stimulation-Expressed

Species: Human

Assay Type: Sandwich

Sensitivity: 22.3 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Like Protein 1 (IL1RL1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Receptor Like Protein 1 (IL1RL1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Like Protein 1 (IL1RL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Like Protein 1 (IL1RL1) in the samples is then determined by comparing the OD of the samples to the standard curve.