Human IL4R (Interleukin 4 Receptor) ELISA Kit | ELK1785

Human IL4R (Interleukin 4 Receptor) ELISA Kit | ELK1785 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-4R;CD124; IL4-R; IL4RA; IL4-BP; Soluble interleukin-4 receptor subunit alpha; IL-4-binding protein

Species: Human

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Rheumatology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 Receptor (IL4R) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 4 Receptor (IL4R) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 Receptor (IL4R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 4 Receptor (IL4R) in the samples is then determined by comparing the OD of the samples to the standard curve.