Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit | KTE62407

Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit | KTE62407 | Gentaur UK, US & Europe Distribution

Application: This Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP10 in samples. An antibody specific for DPP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Inactive dipeptidyl peptidase 10 microplate
• Human Inactive dipeptidyl peptidase 10 standard
• Human Inactive dipeptidyl peptidase 10 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPP10. No significant cross-reactivity or interference between Human DPP10 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DPP10 encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. DPP10 lacks the active-site serine, which is substituted with a glycine residue. Database analysis suggested the presence of a second DPP10 transcript. DPP10 shares 48% and 51% amino acid identity with the short and long DPP6 isoforms, respectively.

Alternative Names: DPP10; DPL2; DPPY; DPRP3; OTTHUMP00000203618; dipeptidyl peptidase 10; dipeptidyl peptidase IV-related protein 3; dipeptidyl peptidase X; dipeptidyl peptidase like protein 2

Search name: DPP10; DPL2; DPPY; DPRP3; OTTHUMP00000203618; dipeptidyl peptidase 10; dipeptidyl peptidase IV-related protein 3; dipeptidyl peptidase X; dipeptidyl peptidase like protein 2

Tag: DPP10