Human INHbA (Inhibin Beta A) ELISA Kit | ELK5311

Human INHbA (Inhibin Beta A) ELISA Kit | ELK5311 | Gentaur UK, US & Europe Distribution

Alternative Names: INH-BA; INHB-A; EDF; FRP; Activin A, Activin AB Alpha Polypeptide; Activin beta-A chain; Erythroid differentiation protein

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.68 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Tumor immunity;Endocrinology;Reproductive science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Inhibin Beta A (INHbA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inhibin Beta A (INHbA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibin Beta A (INHbA) in the samples is then determined by comparing the OD of the samples to the standard curve.