Human INPP4A (Inositol Polyphosphate-4-Phosphatase Type I 107kDa) ELISA Kit | ELK6108

Human INPP4A (Inositol Polyphosphate-4-Phosphatase Type I 107kDa) ELISA Kit | ELK6108 | Gentaur UK, US & Europe Distribution

Alternative Names: INPP4; Inositol polyphosphate 4-phosphatase type I; Type I inositol 3, 4-bisphosphate 4-phosphatase

Species: Human

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) in the samples is then determined by comparing the OD of the samples to the standard curve.