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Human INPP5D (Inositol Polyphosphate-5-Phosphatase 145kDa) ELISA Kit | ELK7560
- SKU:
- 855-ELK7560-GEN
Description
Human INPP5D (Inositol Polyphosphate-5-Phosphatase 145kDa) ELISA Kit | ELK7560 | Gentaur UK, US & Europe Distribution
Alternative Names: SHIP; SHIP1; SIP-145; hp51CN; Phosphatidylinositol-3, 4, 5-Trisphosphate 5-Phosphatase 1; SH2 domain-containing inositol 5'-phosphatase 1
Species: Human
Assay Type: Sandwich
Sensitivity: 13.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) in the samples is then determined by comparing the OD of the samples to the standard curve.