Human Intercellular adhesion molecule 4 (ICAM4) ELISA Kit | KTE62384

Human Intercellular adhesion molecule 4 (ICAM4) ELISA Kit | KTE62384 | Gentaur UK, US & Europe Distribution

Application: This Human Intercellular adhesion molecule 4 (ICAM4) ELISA Kit employs a two-site sandwich ELISA to quantitate ICAM4 in samples. An antibody specific for ICAM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyICAM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ICAM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ICAM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Intercellular adhesion molecule 4 microplate
• Human Intercellular adhesion molecule 4 standard
• Human Intercellular adhesion molecule 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Intercellular adhesion molecule 4 (ICAM4) ELISA Kit has high sensitivity and excellent specificity for detection of Human ICAM4. No significant cross-reactivity or interference between Human ICAM4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The LW glycoprotein has recently been renamed ICAM-4 due to its similarity to intercellular adhesion molecule, although exactly which integrins bind to ICAM-4 is subject to controversy. The function of ICAM-4 is not fully understood but appears to be restricted to erythroid cells. During in vitro erythropoesis, LW appears at either the erythroid colony forming stage or later at the proerythroblast stage. A vital part of erythropoesis is the clustering of erythroblasts around bone marrow macrophages to form erythroblastic islands. The erythroblast is then able to remove its nucleus, which is in turn ingested and broken down by the macrophages, to become a mature erythrocyte. During this process ICAM-4 binds to VLA-4, an erythroblast binding site, on adjacent erythroblasts and to alpha integrins on macrophages to help stabilise the erythroblastic islands.

Alternative Names: ICAM4; CD242; LW; CD242 antigen; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; intercellular adhesion molecule 4; intercellular adhesion molecule 4 (LW blood group)

Search name: ICAM4; CD242; LW; CD242 antigen; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; intercellular adhesion molecule 4; intercellular adhesion molecule 4 (LW blood group)

Tag: ICAM4