Human Interferon alpha antibody (IFNA-Ab) ELISA Kit | AE62568HU

Human Interferon alpha antibody (IFNA-Ab) ELISA Kit | AE62568HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: IFNA-Ab

Alternative Name: N/A

Application: ELISA

Range: 7.8-500 pg/mL

Sensitivity: 3.0 pg/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤7.6%

Recovery: 0, 95

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Qualitative

Test Principale: This assay employs a two-site sandwich ELISA to quantitate IFNA-Ab in samples. An antibody specific for IFNA-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFNA-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNA-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNA-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Streuli et al. (1980) showed that at least 3 different IFN-alpha genes are expressed in man. Furthermore, study of genomic DNA revealed the presence of at least 8 IFN genes.Nagata et al. (1980) found that the alpha-interferon genes are devoid of intervening sequences. Using radioactive probes from purified cDNA clones of interferons, Owerbach et al. (1981) located at least 8 leukocyte interferon genes and a fibroblast interferon gene on chromosome 9. Shows et al. (1982) found that the alpha- and beta-interferon genes are on 9p. The mapping to 9pter-q12 was accomplished by blot hybridization of cloned interferon cDNA to DNA from human-mouse cell hybrids with a translocation involving chromosome 9.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .