Human Interleukin-36 gamma (IL1F9) ELISA Kit | KTE62244

Human Interleukin-36 gamma (IL1F9) ELISA Kit | KTE62244 | Gentaur UK, US & Europe Distribution

Application: This Human Interleukin-36 gamma (IL1F9) ELISA Kit employs a two-site sandwich ELISA to quantitate IL1F9 in samples. An antibody specific for IL1F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL1F9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Interleukin-36 gamma microplate
• Human Interleukin-36 gamma standard
• Human Interleukin-36 gamma detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Interleukin-36 gamma (IL1F9) ELISA Kit has high sensitivity and excellent specificity for detection of Human IL1F9. No significant cross-reactivity or interference between Human IL1F9 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: IL1F9 encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2, and is specifically inhibited by interleukin 1 family, member 5 . Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.

Alternative Names: IL1F9; IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; IL-1 related protein 2; IL-1 (EPSILON) ; IL-1-epsilon; interleukin 1-related protein 2; interleukin-1 epsilon; interleukin-1 homolog 1

Search name: IL1F9; IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; IL-1 related protein 2; IL-1 (EPSILON) ; IL-1-epsilon; interleukin 1-related protein 2; interleukin-1 epsilon; interleukin-1 homolog 1

Tag: IL1F9