Human IRF9 (Interferon Regulatory Factor 9) ELISA Kit | ELK3224

Human IRF9 (Interferon Regulatory Factor 9) ELISA Kit | ELK3224 | Gentaur UK, US & Europe Distribution

Alternative Names: ISGF3G; ISGF3; p48; Interferon-Stimulated Transcription Factor 3, Gamma 48kDa; IFN-alpha-responsive transcription factor subunit; Interferon-stimulated gene factor 3 gamma

Species: Human

Assay Type: Sandwich

Sensitivity: 0.048 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 9 (IRF9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Regulatory Factor 9 (IRF9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 9 (IRF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Regulatory Factor 9 (IRF9) in the samples is then determined by comparing the OD of the samples to the standard curve.