Human ITaC (Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit | ELK1344

Human ITaC (Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit | ELK1344 | Gentaur UK, US & Europe Distribution

Alternative Names: CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine (C-X-C-Motif) ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9

Species: Human

Assay Type: Sandwich

Sensitivity: 24.2 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Signal transduction;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible T-Cell Alpha Chemoattractant (ITaC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) in the samples is then determined by comparing the OD of the samples to the standard curve.