Human ITGb1 (Integrin Beta 1) ELISA Kit | ELK3225

Human ITGb1 (Integrin Beta 1) ELISA Kit | ELK3225 | Gentaur UK, US & Europe Distribution

Alternative Names: CD29; MSK12; ITG-B1; FNRB; GPIIA; MDF2; MSK12; VLAB; Fibronectin Receptor Beta Polypeptide; Antigen CD29 Includes MDF2; Glycoprotein IIa; VLA-4 subunit beta

Species: Human

Assay Type: Sandwich

Sensitivity: 0.35 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 1 (ITGb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Integrin Beta 1 (ITGb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 1 (ITGb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 1 (ITGb1) in the samples is then determined by comparing the OD of the samples to the standard curve.