Human ITIH2 (Inter Alpha-Globulin Inhibitor H2) ELISA Kit | ELK3441

Human ITIH2 (Inter Alpha-Globulin Inhibitor H2) ELISA Kit | ELK3441 | Gentaur UK, US & Europe Distribution

Alternative Names: H2P; SHAP; IGHEP2; Serum-derived hyaluronan-associated protein; Inter-alpha-trypsin inhibitor complex component II

Species: Human

Assay Type: Sandwich

Sensitivity: 1.42 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H2 (ITIH2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H2 (ITIH2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H2 (ITIH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inter Alpha-Globulin Inhibitor H2 (ITIH2) in the samples is then determined by comparing the OD of the samples to the standard curve.