Human ITIH4 (Inter Alpha-Globulin Inhibitor H4) ELISA Kit | ELK3097

Human ITIH4 (Inter Alpha-Globulin Inhibitor H4) ELISA Kit | ELK3097 | Gentaur UK, US & Europe Distribution

Alternative Names: IHRP; PK120; H4P; ITIHL1; Plasma Kallikrein-Sensitive Glycoprotein; Plasma kallikrein sensitive glycoprotein 120; Inter-alpha-trypsin inhibitor family heavy chain-related protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.17 ng/mL

Standard: 25 ng/mL

Detection Range: 0.4-25 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H4 (ITIH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inter Alpha-Globulin Inhibitor H4 (ITIH4) in the samples is then determined by comparing the OD of the samples to the standard curve.