Human JUN (V-Jun Sarcoma Virus 17 Oncogene Homolog) ELISA Kit | ELK9051

Human JUN (V-Jun Sarcoma Virus 17 Oncogene Homolog) ELISA Kit | ELK9051 | Gentaur UK, US & Europe Distribution

Alternative Names: C-Jun; AP1; CJun; p39; Jun Oncogene; Proto-oncogene c-Jun; Activator protein 1; Transcription factor AP-1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) in the samples is then determined by comparing the OD of the samples to the standard curve.