Human Kallikrein 1 (KLK1) ELISA Kit | KTE61903

Human Kallikrein 1 (KLK1) ELISA Kit | KTE61903 | Gentaur UK, US & Europe Distribution

Application: This Human Kallikrein 1 (KLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK1 in samples. An antibody specific for KLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kallikrein 1 microplate
• Human Kallikrein 1 standard
• Human Kallikrein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kallikrein 1 (KLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK1. No significant cross-reactivity or interference between Human KLK1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KLK1, or tissue kallikrein is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. Kallikrein 1 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. the deduced 262-amino acid KLK1 protein contains a putative signal peptide, followed by a short activating peptide and the protease domain. The protease domain contains the catalytic triad of his65, asp120, and ser214. RT-PCR detected variable expression of KLK1 in most of the 35 tissues examined. Highest expression was in pancreas, salivary gland, thyroid, parotid gland, fetal and adult skin, kidney, and liver.

Alternative Names: KLK1; KLKR; Klk6; hK1; glandular kallikrein 1; kallikrein 1; renal/pancreas/salivary; kallikrein serine protease 1; tissue kallikrein

Search name: KLK1; KLKR; Klk6; hK1; glandular kallikrein 1; kallikrein 1; renal/pancreas/salivary; kallikrein serine protease 1; tissue kallikrein

Tag: KLK1