Human Kallikrein 10 (KLK10) ELISA Kit | KTE61902

Human Kallikrein 10 (KLK10) ELISA Kit | KTE61902 | Gentaur UK, US & Europe Distribution

Application: This Human Kallikrein 10 (KLK10) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK10 in samples. An antibody specific for KLK10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kallikrein 10 microplate
• Human Kallikrein 10 standard
• Human Kallikrein 10 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kallikrein 10 (KLK10) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK10. No significant cross-reactivity or interference between Human KLK10 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KLK10 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein. The deduced 276-amino acid protein contains a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his86, asp137, and ser229. KLK10 also contains 12 conserved cysteines in its protease domain that are expected to form 6 disulfide bonds, as in other serine proteases. RT-PCR detected variable levels of KLK10 expression in nearly all of the 35 adult and fetal tissues examined.

Alternative Names: KLK10; NES1; PRSSL1; breast normal epithelial cell associated serine protease; kallikrein 10; normal epithelial cell-specific 1; protease; serine-like; 1

Search name: KLK10; NES1; PRSSL1; breast normal epithelial cell associated serine protease; kallikrein 10; normal epithelial cell-specific 1; protease; serine-like; 1

Tag: KLK10