Human Kallikrein 11 (KLK11) ELISA Kit | KTE61901

Human Kallikrein 11 (KLK11) ELISA Kit | KTE61901 | Gentaur UK, US & Europe Distribution

Application: This Human Kallikrein 11 (KLK11) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK11 in samples. An antibody specific for KLK11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kallikrein 11 microplate
• Human Kallikrein 11 standard
• Human Kallikrein 11 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kallikrein 11 (KLK11) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK11. No significant cross-reactivity or interference between Human KLK11 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Kallikrein 11 is a member of the human kallikrein gene family. KLK11 is a secreted serine protease, highly expressed in hormonally regulated tissues, including the prostate and the ovary. KLK11 has two alternative splicing isoforms, known as the brain type and prostate type. Many members of the human kallikrein gene family are differentially expressed in cancer and a few have potential as diagnostic/prognostic markers. KLK11 may represent a diagnostic and prognostic biomarker for ovarian cancer. KLK11 expression in prostate, testis, trachea, pancreas, salivary gland, thyroid, parathyroid, lung, stomach, and adult and fetal skin. Weaker expression was detected in heart, mammary gland, uterus, colon, and small intestine, with little to no expression in other tissues examined.

Alternative Names: KLK11; MGC33060; PRSS20; TLSP; hippostasin; kallikrein 11; protease; serine; 20 trypsin-like; protease; serine; trypsin-like

Search name: KLK11; MGC33060; PRSS20; TLSP; hippostasin; kallikrein 11; protease; serine; 20 trypsin-like; protease; serine; trypsin-like

Tag: KLK11