Human Kallikrein-13 (KLK13) ELISA Kit | KTE61899

Human Kallikrein-13 (KLK13) ELISA Kit | KTE61899 | Gentaur UK, US & Europe Distribution

Application: This Human Kallikrein-13 (KLK13) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK13 in samples. An antibody specific for KLK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kallikrein-13 microplate
• Human Kallikrein-13 standard
• Human Kallikrein-13 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kallikrein-13 (KLK13) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK13. No significant cross-reactivity or interference between Human KLK13 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Kallikrein-13 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Expression of this gene is regulated by steroid hormones and may be useful as a marker for breast cancer. An additional transcript variant has been identified, but its full length sequence has not been determined. in a 300-kb region on chromosome 19q13.3-q13.4, where other kallikrein genes are localized. KLK13 exists in 2 main forms: a long form that encodes a deduced 277-amino acid protein with a predicted molecular mass of 30.6 kD, and a short form, referred to as the short KLKL4 variant, that uses an upstream alternative splice donor site located in exon 3 that generates a truncated protein. The KLK13 protein shares 51% amino acid identity with KLK11 and KLK6.

Alternative Names: KLK13; DKFZp586J1923; KLK-L4; KLKL4; kallikrein 13; kallikrein-like gene 4

Search name: KLK13; DKFZp586J1923; KLK-L4; KLKL4; kallikrein 13; kallikrein-like gene 4

Tag: KLK13