Human KCC4 (Potassium Chloride Cotransporters 4) ELISA Kit | ELK2134

Human KCC4 (Potassium Chloride Cotransporters 4) ELISA Kit | ELK2134 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC12A7; Solute Carrier Family 12 (Potassium/Chloride Transporters) Member 7; Electroneutral potassium-chloride cotransporter 4; K-Cl cotransporter 4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Chloride Cotransporters 4 (KCC4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Potassium Chloride Cotransporters 4 (KCC4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Chloride Cotransporters 4 (KCC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Potassium Chloride Cotransporters 4 (KCC4) in the samples is then determined by comparing the OD of the samples to the standard curve.