Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit | KTE61879

Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit | KTE61879 | Gentaur UK, US & Europe Distribution

Application: This Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT33A in samples. An antibody specific for KRT33A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT33A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT33A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT33A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratin, type I cuticular Ha3-I microplate
• Human Keratin, type I cuticular Ha3-I standard
• Human Keratin, type I cuticular Ha3-I detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratin, type I cuticular Ha3-I (KRT33A) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT33A. No significant cross-reactivity or interference between Human KRT33A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KRT33A is a member of the keratin gene family. It is one of the type I hair keratin genes which are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B. Rogers et al. (1998) reported that the deduced KRTHA3A protein has 405 amino acids and shares 93.3% amino acid sequence identity with KRTHA3B. By RT-PCR, Rogers et al. (1998) showed that KRTHA3A is expressed in the human hair follicle. See Langbein et al. (1999) for further details on the expression pattern of the KRTHA3A gene in the hair follicle.

Alternative Names: KRT33A; HA3I; Ha-3I; KRTHA3A; Krt1-3; hHa3-I; hard keratin; type I;3I; keratin; hair; acidic; 3A

Search name: KRT33A; HA3I; Ha-3I; KRTHA3A; Krt1-3; hHa3-I; hard keratin; type I;3I; keratin; hair; acidic; 3A

Tag: KRT33A