Human Keratin, type I cytoskeletal 16 (KRT16) ELISA Kit | KTE61883

Human Keratin, type I cytoskeletal 16 (KRT16) ELISA Kit | KTE61883 | Gentaur UK, US & Europe Distribution

Application: This Human Keratin, type I cytoskeletal 16 (KRT16) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT16 in samples. An antibody specific for KRT16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratin, type I cytoskeletal 16 microplate
• Human Keratin, type I cytoskeletal 16 standard
• Human Keratin, type I cytoskeletal 16 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratin, type I cytoskeletal 16 (KRT16) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT16. No significant cross-reactivity or interference between Human KRT16 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Keratin 16 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.

Alternative Names: KRT16; CK16; FNEPPK; K16; K1CP; KRT16A; NEPPK; cytokeratin 16; focal non-epidermolytic palmoplantar keratoderma; keratin; type I cytoskeletal 16

Search name: KRT16; CK16; FNEPPK; K16; K1CP; KRT16A; NEPPK; cytokeratin 16; focal non-epidermolytic palmoplantar keratoderma; keratin; type I cytoskeletal 16

Tag: KRT16