Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit | KTE61872

Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit | KTE61872 | Gentaur UK, US & Europe Distribution

Application: This Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT81 in samples. An antibody specific for KRT81 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT81 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT81 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT81 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratin, type II cuticular Hb1 microplate
• Human Keratin, type II cuticular Hb1 standard
• Human Keratin, type II cuticular Hb1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratin, type II cuticular Hb1 (KRT81) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT81. No significant cross-reactivity or interference between Human KRT81 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KRT81 is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The predicted 495-amino acid HB1 protein has an 88-amino acid C-terminal domain. The amino acid sequences of human and sheep HB1 are 90% identical. Northern blot analysis detected HB1 transcripts in human scalp but not unhaired breast epidermis. HB1 is more closely related to HB3 and HB6 than to HB5. In situ hybridization studies demonstrated that type II keratin genes are sequentially activated in the hair follicle in the following order: HB5-- (HB1, HB3) --HB6. Both HB1 and HB3 mRNA syntheses begin 10 to 15 cell layers above the apex of the dermal papilla and end abruptly in the middle of the cortex.

Alternative Names: KRT81; HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1; hard keratin; type; 1; keratin; hair; basic; 1

Search name: KRT81; HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1; hard keratin; type; 1; keratin; hair; basic; 1

Tag: KRT81