Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit | KTE61888

Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit | KTE61888 | Gentaur UK, US & Europe Distribution

Application: This Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT1 in samples. An antibody specific for KRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratin, type II cytoskeletal 1 microplate
• Human Keratin, type II cytoskeletal 1 standard
• Human Keratin, type II cytoskeletal 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT1. No significant cross-reactivity or interference between Human KRT1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.Keratin 1 is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants of bullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected.

Alternative Names: KRT1; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; cytokeratin 1; epidermolytic hyperkeratosis 1; hair alpha protein; keratin; type cytoskeletal 1

Search name: KRT1; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; cytokeratin 1; epidermolytic hyperkeratosis 1; hair alpha protein; keratin; type cytoskeletal 1

Tag: KRT1