Human Keratin, type II cytoskeletal 4 (KRT4) ELISA Kit | KTE61878

Human Keratin, type II cytoskeletal 4 (KRT4) ELISA Kit | KTE61878 | Gentaur UK, US & Europe Distribution

Application: This Human Keratin, type II cytoskeletal 4 (KRT4) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT4 in samples. An antibody specific for KRT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratin, type II cytoskeletal 4 microplate
• Human Keratin, type II cytoskeletal 4 standard
• Human Keratin, type II cytoskeletal 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratin, type II cytoskeletal 4 (KRT4) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT4. No significant cross-reactivity or interference between Human KRT4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Keratin 4 isa member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]. Sequence Note: The RefSeq represents one of two co-dominant alleles identified for this gene.

Alternative Names: KRT4; CK4; CYK4; FLJ31692; K4; cytokeratin 4; keratin; type cytoskeletal 4

Search name: KRT4; CK4; CYK4; FLJ31692; K4; cytokeratin 4; keratin; type cytoskeletal 4

Tag: KRT4