Human Keratin, type II cytoskeletal 5 (KRT5) ELISA Kit | KTE61876

Human Keratin, type II cytoskeletal 5 (KRT5) ELISA Kit | KTE61876 | Gentaur UK, US & Europe Distribution

Application: This Human Keratin, type II cytoskeletal 5 (KRT5) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT5 in samples. An antibody specific for KRT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratin, type II cytoskeletal 5 microplate
• Human Keratin, type II cytoskeletal 5 standard
• Human Keratin, type II cytoskeletal 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratin, type II cytoskeletal 5 (KRT5) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT5. No significant cross-reactivity or interference between Human KRT5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic (i.e., different types of) keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. The type II cytokeratin genes are clustered in a region of chromosome 12q12-q13. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Eckert and Rorke (1988) cloned full-length KRT5 from a cultured keratinocyte cDNA library. The deduced 590-amino acid protein contains a central alpha-helical core region of about 310 amino acids with a 7-amino acid repeat found in coiled-coil structures. The N and C termini are serine rich and contain gly-gly-gly-x motifs.

Alternative Names: KRT5; CK5; DDD; EBS2; K5; KRT5A; 58 kda cytokeratin; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex; Dowling-Meara/Kobner/Weber-Cockayne

Search name: KRT5; CK5; DDD; EBS2; K5; KRT5A; 58 kda cytokeratin; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex; Dowling-Meara/Kobner/Weber-Cockayne

Tag: KRT5