Human Keratocan (KERA) ELISA Kit | KTE61923

Human Keratocan (KERA) ELISA Kit | KTE61923 | Gentaur UK, US & Europe Distribution

Application: This Human Keratocan (KERA) ELISA Kit employs a two-site sandwich ELISA to quantitate KERA in samples. An antibody specific for KERA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKERA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KERA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KERA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Keratocan microplate
• Human Keratocan standard
• Human Keratocan detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Keratocan (KERA) ELISA Kit has high sensitivity and excellent specificity for detection of Human KERA. No significant cross-reactivity or interference between Human KERA and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Keratocan is a protein encoded by the KERA gene.Keratan sulfate proteoglycans (KSPGs) are members of the small leucine-rich proteoglycan (SLRP) family. KSPGs, particularly keratocan, lumican, and mimecan, are important to the transparency of the cornea. Defects in KERA are the cause of the autosomal recessive cornea plana 2 (CNA2) . In CNA2, the forward convex curvature is flattened, leading to a decrease in refraction, reduced visual activity, extreme hyperopia (usually plus 10 d or more), hazy corneal limbus, opacities in the corneal parenchyma, and marked arcus senilis (often detected at an early age) . CNA2 is a rare disorder with a worldwide distribution, but a high prevalence in the Finnish population.

Alternative Names: KERA; CNA2; SLRR2B; cornea plana 2 (autosomal recessive) ; keratocan proteoglycan

Search name: KERA; CNA2; SLRR2B; cornea plana 2 (autosomal recessive) ; keratocan proteoglycan

Tag: KERA