Human KIF20A (Kinesin Family, Member 20A) ELISA Kit | ELK3684

Human KIF20A (Kinesin Family, Member 20A) ELISA Kit | ELK3684 | Gentaur UK, US & Europe Distribution

Alternative Names: MKLP2; RAB6KIFL; GG10_2; RAB6 Interacting, Kinesin-Like; Rab Kinesin 6; Mitotic kinesin-like protein 2; Rabkinesin-6

Species: Human

Assay Type: Sandwich

Sensitivity: 0.049 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 20A (KIF20A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kinesin Family, Member 20A (KIF20A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 20A (KIF20A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 20A (KIF20A) in the samples is then determined by comparing the OD of the samples to the standard curve.