Human KIF5A (Kinesin Family, Member 5A) ELISA Kit | ELK3930

Human KIF5A (Kinesin Family, Member 5A) ELISA Kit | ELK3930 | Gentaur UK, US & Europe Distribution

Alternative Names: NKHC; MY050; SPG10; Spastic Paraplegia 10 (Autosomal Dominant) ; Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain

Species: Human

Assay Type: Sandwich

Sensitivity: 0.112 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5A (KIF5A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kinesin Family, Member 5A (KIF5A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 5A (KIF5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 5A (KIF5A) in the samples is then determined by comparing the OD of the samples to the standard curve.