Human Kinectin (KTN1) ELISA Kit | KTE61871

Human Kinectin (KTN1) ELISA Kit | KTE61871 | Gentaur UK, US & Europe Distribution

Application: This Human Kinectin (KTN1) ELISA Kit employs a two-site sandwich ELISA to quantitate KTN1 in samples. An antibody specific for KTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kinectin microplate
• Human Kinectin standard
• Human Kinectin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kinectin (KTN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KTN1. No significant cross-reactivity or interference between Human KTN1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Kinectin is a protein encoded by the KTN1 gene.Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule organizing center, and alignment of lysosomes along microtubules are all related processes. The transport of organelles requires a special class of microtubule-associated proteins (MAPs) . One of these is the molecular motor kinesin, an ATPase that moves vesicles unidirectionally toward the plus end of the microtubule. Another such MAP is kinectin, a large integral ER membrane protein. Antibodies directed against kinectin have been shown to inhibit its binding to kinesin.

Alternative Names: KTN1; CG1; KIAA0004; KNT; MGC133337; MU-RMS-40.19; CG-1 antigen; OTTHUMP00000179011; kinectin 1; kinesin receptor

Search name: KTN1; CG1; KIAA0004; KNT; MGC133337; MU-RMS-40.19; CG-1 antigen; OTTHUMP00000179011; kinectin 1; kinesin receptor

Tag: KTN1