Human Kinesin heavy chain isoform 5A (KIF5A) ELISA Kit | KTE61916

Human Kinesin heavy chain isoform 5A (KIF5A) ELISA Kit | KTE61916 | Gentaur UK, US & Europe Distribution

Application: This Human Kinesin heavy chain isoform 5A (KIF5A) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF5A in samples. An antibody specific for KIF5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kinesin heavy chain isoform 5A microplate
• Human Kinesin heavy chain isoform 5A standard
• Human Kinesin heavy chain isoform 5A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kinesin heavy chain isoform 5A (KIF5A) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIF5A. No significant cross-reactivity or interference between Human KIF5A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The predicted 1, 032-amino acid protein has the characteristic features of a KHC, as well as a unique C-terminal stretch of 69 amino acids. The amino acid sequence of NKHC is 65% and 54% identical to the amino acid sequences of KNS1 and the Drosophila KHC. Northern blot analysis of rat tissues detected Nkhc expression only in brain, where multiple transcripts were found. Immunoblot analysis of rat tissue extracts using antibodies specific for NKHC detected a doublet of approximately 120 and 133 kD only in brain and sciatic nerve tissue. By indirect immunofluorescence, the authors showed that NKHC is distributed throughout the central nervous system but is highly enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC is concentrated in the cell body, particularly in the perinuclear region.

Alternative Names: KIF5A; D12S1889; MY050; NKHC; SPG10; KIF5A variant protein; kinesin; heavy chain; neuron-specific

Search name: KIF5A; D12S1889; MY050; NKHC; SPG10; KIF5A variant protein; kinesin; heavy chain; neuron-specific

Tag: KIF5A