Human Kinesin-like protein KIF1A (KIF1A) ELISA Kit | KTE61918

Human Kinesin-like protein KIF1A (KIF1A) ELISA Kit | KTE61918 | Gentaur UK, US & Europe Distribution

Application: This Human Kinesin-like protein KIF1A (KIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF1A in samples. An antibody specific for KIF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kinesin-like protein KIF1A microplate
• Human Kinesin-like protein KIF1A standard
• Human Kinesin-like protein KIF1A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kinesin-like protein KIF1A (KIF1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIF1A. No significant cross-reactivity or interference between Human KIF1A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KIF1A is a member of the kinesin family. This protein is highly similar to mouse heavy chain kinesin member 1A protein which is an anterograde motor protein that transports membranous organelles along axonal microtubules. It is thought that this protein may play a critical role in the development of axonal neuropathies resulting from impaired axonal transport. There are multiple polyadenylation sites found in this gene. This switch-like transition for movement could be shifted to lower PtdIns (4, 5) P2 concentrations by the addition of cholesterol/sphingomyelin or GM1 ganglioside/cholera toxin, conditions that produced raft-like behavior of Unc104 bound to lipid bilayers. The authors concluded that clustering of Unc104 in PtdIns (4, 5) P2-containing rafts provides a trigger for membrane transport.

Alternative Names: KIF1A; ATSV; C2orf20; DKFZp686I2094; FLJ30229; HUNC-104; MGC133285; MGC133286; UNC104; axonal transport of synaptic vesicles; kinesin; heavy chain; member 1A; homolog of mouse; tmp_locus_27

Search name: KIF1A; ATSV; C2orf20; DKFZp686I2094; FLJ30229; HUNC-104; MGC133285; MGC133286; UNC104; axonal transport of synaptic vesicles; kinesin; heavy chain; member 1A; homolog of mouse; tmp_locus_27

Tag: KIF1A