Human KLK1 (Kallikrein 1) ELISA Kit | ELK4888

Human KLK1 (Kallikrein 1) ELISA Kit | ELK4888 | Gentaur UK, US & Europe Distribution

Alternative Names: KLKR; Klk6; HK1; Kallikrein 1, Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 27.51 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Tumor immunity;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 1 (KLK1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 1 (KLK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 1 (KLK1) in the samples is then determined by comparing the OD of the samples to the standard curve.