Human KLK10 (Kallikrein 10) ELISA Kit | ELK1110

Human KLK10 (Kallikrein 10) ELISA Kit | ELK1110 | Gentaur UK, US & Europe Distribution

Alternative Names: NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 48.3 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Tumor immunity;Reproductive science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 10 (KLK10) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 10 (KLK10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 10 (KLK10) in the samples is then determined by comparing the OD of the samples to the standard curve.