Human KLK8 (Kallikrein 8) ELISA Kit | ELK1851

Human KLK8 (Kallikrein 8) ELISA Kit | ELK1851 | Gentaur UK, US & Europe Distribution

Alternative Names: NP; HNP; NRPN; PRSS19; TADG14; BSP1; Neuropsin; Ovasin; Brain Serine Protease 1; Tumor Associated Differentially Expressed Gene 14; Serine protease 19

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 225.3 pg/mL

Standard: 50000 pg/mL

Detection Range: 781.25-50000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Tumor immunity;Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 8 (KLK8) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 8 (KLK8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 8 (KLK8) in the samples is then determined by comparing the OD of the samples to the standard curve.