Human KPNa2 (Karyopherin Alpha 2) ELISA Kit | ELK4114

Human KPNa2 (Karyopherin Alpha 2) ELISA Kit | ELK4114 | Gentaur UK, US & Europe Distribution

Alternative Names: IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha

Species: Human

Assay Type: Sandwich

Sensitivity: 52 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Karyopherin Alpha 2 (KPNa2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Alpha 2 (KPNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Alpha 2 (KPNa2) in the samples is then determined by comparing the OD of the samples to the standard curve.