Human Krebs Von den Lungen-6 (KL-6) ELISA Kit | ELK9353

Human Krebs Von den Lungen-6 (KL-6) ELISA Kit | ELK9353 | Gentaur UK, US & Europe Distribution

Alternative Names: KL-6

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 5.9IU/mL

Standard: 800IU/mL

Detection Range: 12.5-800 IU/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Interstitial lung disease

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with KL-6 (Krebs Von den Lungen-6) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to KL-6 (Krebs Von den Lungen-6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KL-6 (Krebs Von den Lungen-6) in the samples is then determined by comparing the OD of the samples to the standard curve.