Human KRT18 (Keratin 18) ELISA Kit | ELK1816

Human KRT18 (Keratin 18) ELISA Kit | ELK1816 | Gentaur UK, US & Europe Distribution

Alternative Names: CK18; CYK18; K18; Cytokeratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18

Species: Human

Assay Type: Sandwich

Sensitivity: 0.06 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Pulmonology;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Keratin 18 (KRT18) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the OD of the samples to the standard curve.