Human KRT33A (Keratin 33A) ELISA Kit | ELK6748

Human KRT33A (Keratin 33A) ELISA Kit | ELK6748 | Gentaur UK, US & Europe Distribution

Alternative Names: KRTHA3A; Ha-3I; Krt1-3; K33A; HHA3-I; HKA3A; Keratin, Hair, Acidic 3A; Hard Keratin Type I 3I; Keratin, type I cuticular Ha3-I

Species: Human

Assay Type: Sandwich

Sensitivity: 29 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 33A (KRT33A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Keratin 33A (KRT33A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 33A (KRT33A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 33A (KRT33A) in the samples is then determined by comparing the OD of the samples to the standard curve.