Human KRT7 (Keratin 7) ELISA Kit | ELK4022

Human KRT7 (Keratin 7) ELISA Kit | ELK4022 | Gentaur UK, US & Europe Distribution

Alternative Names: CK7; K2C7; K7; SCL; Cytokeratin 7; Sarcolectin; Keratin, 55K Type II Cytoskeletal; Keratin, type II cytoskeletal 7

Species: Human

Assay Type: Sandwich

Sensitivity: 28 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 7 (KRT7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Keratin 7 (KRT7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 7 (KRT7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 7 (KRT7) in the samples is then determined by comparing the OD of the samples to the standard curve.