Human KRT81 (Keratin 81) ELISA Kit | ELK6557

Human KRT81 (Keratin 81) ELISA Kit | ELK6557 | Gentaur UK, US & Europe Distribution

Alternative Names: KRTHB1; Hb-1; K81; MLN137; Keratin, Hair, Basic 1; Hard Keratin Type II 1; Keratin, type II cuticular Hb1; Metastatic lymph node 137 gene protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 81 (KRT81) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Keratin 81 (KRT81) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 81 (KRT81), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 81 (KRT81) in the samples is then determined by comparing the OD of the samples to the standard curve.