Human Krueppel-like factor 15 (KLF15) ELISA Kit | KTE61906

Human Krueppel-like factor 15 (KLF15) ELISA Kit | KTE61906 | Gentaur UK, US & Europe Distribution

Application: This Human Krueppel-like factor 15 (KLF15) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF15 in samples. An antibody specific for KLF15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Krueppel-like factor 15 microplate
• Human Krueppel-like factor 15 standard
• Human Krueppel-like factor 15 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Krueppel-like factor 15 (KLF15) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLF15. No significant cross-reactivity or interference between Human KLF15 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KLF15 protein is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.

Alternative Names: KLF15; DKFZp779M1320; KKLF; kidney-enriched Kruppel-like factor

Search name: KLF15; DKFZp779M1320; KKLF; kidney-enriched Kruppel-like factor

Tag: KLF15