Human KYN (Kynurenine) ELISA Kit | ELK9026

Human KYN (Kynurenine) ELISA Kit | ELK9026 | Gentaur UK, US & Europe Distribution

Alternative Names: KYN; Kynurenine

Species: Human

Assay Type: Sandwich

Sensitivity: 3 pmol/ml

Standard: 500 pmol/ml

Detection Range: 7.82-500 pmol/ml

Sample Type: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kynurenine (KYN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kynurenine (KYN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kynurenine (KYN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kynurenine (KYN) in the samples is then determined by comparing the OD of the samples to the standard curve.