Human LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA Kit | ELK5326

Human LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA Kit | ELK5326 | Gentaur UK, US & Europe Distribution

Alternative Names: CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1

Species: Human

Assay Type: Sandwich

Sensitivity: 33 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-1 (LAB7-1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-1 (LAB7-1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-1 (LAB7-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Activation Antigen B7-1 (LAB7-1) in the samples is then determined by comparing the OD of the samples to the standard curve.