Human LAG1 longevity assurance homolog 2 (LASS2) ELISA Kit | KTE61853

Human LAG1 longevity assurance homolog 2 (LASS2) ELISA Kit | KTE61853 | Gentaur UK, US & Europe Distribution

Application: This Human LAG1 longevity assurance homolog 2 (LASS2) ELISA Kit employs a two-site sandwich ELISA to quantitate LASS2 in samples. An antibody specific for LASS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLASS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LASS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LASS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human LAG1 longevity assurance homolog 2 microplate
• Human LAG1 longevity assurance homolog 2 standard
• Human LAG1 longevity assurance homolog 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human LAG1 longevity assurance homolog 2 (LASS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LASS2. No significant cross-reactivity or interference between Human LASS2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LASS2 has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. The deduced 230-amino acid protein is 29% identical to LASS1 over 224 amino acids. It has 4 transmembrane helices (2 fewer than most Lag1 homologs), a Lag1 motif, and a C-terminal acidic domain. Northern blot analysis revealed expression of a 2.4-kb transcript in kidney and liver, with lower levels in brain, heart, placenta, and lung.Expression of LASS2 in hepatocellular carcinoma cell lines led to suppression of cancer cell growth.

Alternative Names: LASS2; CerS2; FLJ10243; L3; MGC987; SP260; TMSG1; LAG1 longevity assurance 2; ceramide synthase 2; longevity assurance (LAG1; S. cerevisiae) homolog 2; tumor metastasis-suppressor

Search name: LASS2; CerS2; FLJ10243; L3; MGC987; SP260; TMSG1; LAG1 longevity assurance 2; ceramide synthase 2; longevity assurance (LAG1; S. cerevisiae) homolog 2; tumor metastasis-suppressor

Tag: LASS2